C5 Protein

Purified human C5 protein


Product Specifications

Citations 9
Description

Purified human C5 protein

Storage Store at -70°C or below. Avoid freeze/thaw.
Form

Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered.

Concentration 1.0 mg/mL (refer to CoA for lot specific concentration)
Applications

See citations and technical data sheet for application info.

Ordering Information

For Research Use Only. Not for use in diagnostic procedures.
Catalog Number A403
Catalog Number (CE) N/A
Size 250 µL
Price (USD) $325.00
Price (EURO) 290,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

Purified human C5 protein

Size 250 µL
Storage

Store at -70°C or below. Avoid freeze/thaw.

Form Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered.
Concentration 1.0 mg/mL (refer to CoA for lot specific concentration)
Applications See citations and technical data sheet for application info.
Purity >95% by SDS-PAGE
Source

Normal human serum

Activity >80% versus normal human serum standard.
Molecular Weight 190,000 Da (2 chains)
Background

Purified human complement component C5. Complement component C5 is unique to the terminal complement pathway. 190 kD C5 protein is comprised of two chains (α 115 kD; ß 75 kD) and is present in plasma at 70 mg/L. Activation of either complement pathway can generate a C5 convertase enzyme capable of cleaving C5 to C5a (a potent anaphylatoxin) and C5b. Together with the other proteins of the terminal pathway, C5b forms the membrane attack complex or MAC.

Citations

Title Year Applications Sample Species Sample Sample Details

C5a-C5AR1 axis as a potential trigger of the rupture of intracranial aneurysms

2024

Complement Digestion Assay

Purified Protein

Purified Protein

Aneurysm

A C5a-Immunoglobulin complex in chronic lymphocytic leukemia patients is associated with decreased complement activity.

2019

ELISA

Human

Plasma

Chronic Lymphocytic Leukemia

A C5a-Immunoglobulin complex in chronic lymphocytic leukemia patients is associated with decreased complement activity.

2019

ELISA

Human

Serum

Chronic Lymphocytic Leukemia

Complement C3a and C5a modulate osteoclast formation and inflammatory response of osteoblasts in synergism with IL-1β

2011

ELISA

Human

Peripheral blood mononuclear cells

CD14 and complement crosstalk and largely mediate the transcriptional response to Escherichia coli in human whole blood as revealed by DNA microarray

2015

Microarray

Human

Whole Blood

Venom from Bothrops lanceolatus, a Snake Species Native to Martinique, Potently Activates the Complement System

2018

Silver Stain

Human

Purified protein

B. lanceolatus venom incubated

A Conformational Change of Complement C5 Is Required for Thrombin-Mediated Cleavage, Revealed by a Novel Ex Vivo Human Whole Blood Model Preserving Full Thrombin Activity.

2021

WB

Human

Purified protein

A serine protease isolated from the bristles of the Amazonic caterpillar, Premolis semirufa, is a potent complement system activator.

2015

Silver Stain

Premolis semirufa (Caterpillar)

Bristle Extract

C5a–C5AR1 axis as a potential trigger of the rupture of intracranial aneurysms

2024

Complement Digestion Assay

Purified Protein

Purified Protein

Aneurysm

P-I snake venom metalloproteinase is able to activate the complement system by direct cleavage of central components of the cascade.

2013

Silver Stain

Snake

Bothrops pirajai venom