The Lyra RSV + hMPV assay is a multiplex real-time PCR assay for the qualitative detection and identification of respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) viral RNA extracted from nasal swabs and nasopharyngeal swab specimens. This real time RSV + hMPV test detects RSV and hMPV and can differentiate between them.
A multiplex real-time RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for RSV, hMPV and the Process Control (PRC). Identification of RSV and hMPV and the PRC occurs using target-specific primers and fluorescent-labeled probes that hybridize to conserved regions in the NS2 and polymerase genes of RSV and polymerase gene of hMPV and the PRC.
The Lyra RSV + hMPV assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra RSV + hMPV RT-PCR is available for use on the Applied Biosystems® 7500 Fast Dx, QuantStudioTM Dx Real-Time PCR Instrument and Cepheid® SmartCycler® II.
The Lyra RSV + hMPV assay is one in a growing menu of tests and intended to aid in the differential diagnosis of RSV and hMPV infections in humans.
Please see chart below for ordering information.
The Lyra RSV + hMPV assay is a multiplex real-time PCR assay for the qualitative detection and identification of respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) viral RNA extracted from nasal swabs and nasopharyngeal swab specimens. This real time RSV + hMPV test detects RSV and hMPV and can differentiate between them.
A multiplex real-time RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for RSV, hMPV and the Process Control (PRC). Identification of RSV and hMPV and the PRC occurs using target-specific primers and fluorescent-labeled probes that hybridize to conserved regions in the NS2 and polymerase genes of RSV and polymerase gene of hMPV and the PRC.
The Lyra RSV + hMPV assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra RSV + hMPV RT-PCR is available for use on the Applied Biosystems® 7500 Fast Dx, QuantStudioTM Dx Real-Time PCR Instrument and Cepheid® SmartCycler® II.
The Lyra RSV + hMPV assay is one in a growing menu of tests and intended to aid in the differential diagnosis of RSV and hMPV infections in humans.
Please see chart below for ordering information.
| Ordering information | ||
| Catalog number | Description | Kit size / case size |
| M103 | Assay Kit | 96 tests |
| M107 | Quidel Molecular RSV + hMPV Control Set | 10 reactions |
Features & benefits
Rehydration solution is simply added to the lyophilized Master Mix for one-step reagent set-up.
Speedy results in less than 70 minutes after extraction.
Simplified and uniform workflow with standard pipetting volumes for easy training.
To access safety data sheets, package inserts, quick reference guides and all other technical and promotional materials, visit our technical documents library.
- Specifications
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| Product specification | |
| Sample type | Throat samples from symptomatic patients |
| Time to results | Results in less than 70 minutes |
| Reagent storage conditions | 2°C to 8°C |
| Controls storage conditions | 2°C to 8°C |
| Sensitivity* | Strep Groups C and G: 95.7% |
| Specificity* | Strep Groups C and G: 98.3% |
| Positive percent agreement* | hMPV: 98.0% |
| Negative percent agreement* | hMPV: 98.9% |
| Sample volume needed for extraction | 180 µL |
| Shelf life | 24 months |
*Data for Applied Biosystems™ 7500 Fast Dx. Refer to Package Insert for additional performance claims.
Workflow using the 7500 series
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