The Lyra Influenza A+B assay is a multiplex real-time RT-PCR assay for the qualitative detection and identification of viral nucleic acids extracted from nasal swabs and nasopharyngeal swabs specimens. The real-time influenza test has been shown to detect HIN1, H3N2 and H7N9 subtypes but does not differentiate the subtypes from Influenza A.
A multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of influenza A occurs using target specific primers and a fluorescent-labeled probe that hybridizes to a conserved region within the matrix protein gene. Identification of influenza B occurs using target specific primers and fluorescent-labeled probes that hybridize to a conserved influenza B sequence within the neuraminidase gene.
The Lyra Influenza A+B assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra Influenza A+B RT-PCR is available for use on the Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument, QuantStudioTM Dx Real-Time PCR Instrument and Cepheid® SmartCycler® II.
The Lyra Influenza A+B assay is one of a growing menu of assays and is intended to aid in the differential diagnosis of influenza A and/or influenza B viral infections in humans.
Please see chart below for ordering information.
The Lyra Influenza A+B assay is a multiplex real-time RT-PCR assay for the qualitative detection and identification of viral nucleic acids extracted from nasal swabs and nasopharyngeal swabs specimens. The real-time influenza test has been shown to detect HIN1, H3N2 and H7N9 subtypes but does not differentiate the subtypes from Influenza A.
A multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of influenza A occurs using target specific primers and a fluorescent-labeled probe that hybridizes to a conserved region within the matrix protein gene. Identification of influenza B occurs using target specific primers and fluorescent-labeled probes that hybridize to a conserved influenza B sequence within the neuraminidase gene.
The Lyra Influenza A+B assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra Influenza A+B RT-PCR is available for use on the Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument, QuantStudioTM Dx Real-Time PCR Instrument and Cepheid® SmartCycler® II.
The Lyra Influenza A+B assay is one of a growing menu of assays and is intended to aid in the differential diagnosis of influenza A and/or influenza B viral infections in humans.
Please see chart below for ordering information.
| Ordering information | ||
| Catalog number | Description | Kit size / Case size |
| M100 | Assay Kit | 96 tests |
| M106 | Quidel Molecular Influenza A+B Control Set | 10 reactions |
Features & benefits
Rehydration solution is simply added to the lyophilized Master Mix for one-step reagent set-up.
Speedy results in less than 75 minutes after extraction.
Simplified and uniform workflow with standard pipetting volumes for easy training.
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- Specifications
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| Product specifications | |
| Sample type | Nasal swab, Nasopharyngeal swab |
| Time to results | Less than 75 minutes after extraction |
| Reagent storage conditions | 2°C to 8°C |
| Controls storage conditions | 2°C to 8°C |
| Positive Percent Agreement* | A: 100%; B: 98.4% |
| Negative Percent Agreement* | A: 98.7%; B: 95.5% |
| LOD | 2 to 100 TCID50/mL |
| Sample volume needed for extraction | 180 µL |
| Purified nucleic acid volume needed to testing | 5 µL |
| Shelf life | 24 months |
*Refer to Package Insert for additional performance claims.
Workflow using the 7500 series
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