iC3b Antibody (Monoclonal - neoantigen) , Biotinylated

A biotin conjugated murine monoclonal antibody to a neo-epitope expressed on iC3b.


Product Specifications

Citations 1
Clonality

Monoclonal

Immunogen Purified human protein.
Applications See citations and technical data sheet for application info.
Concentration 0.2 mg/mL
Conjugate Biotin
Cross Reactivity

Human

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number A710
Catalog Number (CE) N/A
Size 250 µL
Price (USD) $365.00
Price (EURO) 330,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

A biotin conjugated murine monoclonal antibody to a neo-epitope expressed on iC3b.

Size 250 µL
Concentration

0.2 mg/mL

Applications See citations and technical data sheet for application info.
Form Liquid. Phosphate Buffered Saline (pH 7.0 ± 0.2), 1% Bovine Serum Albumin, with ≤ 0.1% Sodium Azide.
Clonality Monoclonal
Immunogen Purified human protein.
Conjugate Biotin
Cross Reactivity Human
Isotype IgG2bk
Purity ≥ 95% by SDS PAGE
Source

Mouse

Specificity This monoclonal antibody was raised against purified human iC3b. It is specific for a neo-antigen on iC3b.
Storage

Short term (30 days) 4˚C. Long term at or below –20˚C.

Background

Under normal conditions, activation of either of the complement pathways leads to the formation of C3 convertase enzymes which cleave C3 into two fragments C3a, an anaphylatoxin, and C3b.The C3b fragment has many biologic functions including promotion of phagocytosis and participation as a structural component in both the C3 and C5 convertase enzymes. These processes are under stringent control in vivo. One control mechanism involves a two-site cleavage of C3b by Factor I with the cooperation of Factor H or CR1 as cofactors. When cleaved in this way the biologic functions of C3b are lost. The resulting protein is termed iC3b.This fragment, in turn, has a host of new biologic activities. iC3b fragments, either in fluid phase or bound to biological surfaces, can interact with a variety of cell types expressing complement receptors (either CR2 or CR3). iC3b levels in fluid phase are elevated in a variety of disease states including Systemic Lupus Erythematosis, Rheumatoid Arthritis as well as in a variety of pathologic conditions including sepsis and Myocardial Infarct. Quidel’s monoclonal antibodies to complement antigens were prepared using standard techniques. They are purified from mouse ascites fluid via protein A affinity chromatography. 3 The specificity of the monoclonal antibody was established via a series of immunological techniques including ELISA, hemagglutination and RIA. Firstly, the antibody was shown by ELISA to bind to C3 antigens using highly pure, immobilized C3. Subsequent studies showed that this antibody agglutinates EC3bi but not EC3b or EC3d cells in an indirect hemagglutination assay. Further experiments showed that this antibody bound to radio-labeled purified iC3b but not to similarly labeled C3, C3b, C3d, or C3c.

Citations

Title Year Applications Sample Species Sample Sample Details

The Carbohydrate-linked Phosphorylcholine of the Parasitic Nematode Product ES-62 Modulates Complement Activation

2016

ELISA

Human

Serum