C4/C4d Antibody (Monoclonal)

A murine monoclonal antibody to human C4d containing fragments.


Product Specifications

Citations3
Clonality

Monoclonal

Immnogen

Purified human protein.

Applications

See citations and technical data sheet for application info.

Concentration1.0-1.2 mg/mL
ConjugateUnconjugated
Cross Reactivity

Human, Pig

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog NumberA253
Catalog Number (CE)N/A
Size500 µl
Price (USD)$1,755.00
Price (EURO)1605,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

A murine monoclonal antibody to human C4d containing fragments.

Size

500 µl

Concentration1.0-1.2 mg/mL
ApplicationsSee citations and technical data sheet for application info.
FormLiquid. Borate Buffered Saline (pH 8.4 ± 0.2), with ≤ 0.1% Sodium Azide.
ClonalityMonoclonal
Immunogen

Purified human protein.

ConjugateUnconjugated
Cross ReactivityHuman, Pig
Isotype

IgG1k

Purity

≥ 95% by SDS PAGE

SourceMouse
Specificity

This monoclonal antibody was raised against purified human C4. It is specific for an antigen expressed on the C4d domain of C4 and therefore reactive to C4 and all C4d-containing fragments of C4.

Storage

Short term (30 days) 4˚C. Long term at or below –20˚C.

Background

Activation of the classical complement pathway begins with the binding of an activating substance (e.g. immune complex) to the C1q molecule of C1. This, in turn, activates the C1r(2)C1s(2) sub-units, resulting in cleavage of C4 to C4b near the amino terminus of the gamma chain releasing C4a in the process. The short-lived C4b molecule can bind covalently to membranes or other surfaces via either an amide or ester bond. This is an inefficient process that is limited to the immediate vicinity of the C1 complex. C4b then takes part in the classical convertase enzyme. Because of the short life of the C4b molecule much of the C4d is free and circulates in serum. Both bound and free C4b are strictly controlled in vivo. The ability of C4b to participate in classical pathway activation and opsonization reactions is inhibited by a single site cleavage of the alpha chain by Factor I. This reaction requires either C4 binding protein or CR1 as a cofactor. This initial cleavage inactivates C4b resulting in iC4b. Further degradation of this molecule by Factor I produces the C4c and C4d fragments. Both of these fragments can be produced in fluid phase or on target surface.