C3/C3c Antibody (Monoclonal)

A murine monoclonal antibody to an epitope in the C3c domain of C3.


Product Specifications

Citations 16
Clonality

Monoclonal

Immunogen Purified human protein.
Applications See citations and technical data sheet for application info.
Concentration ≥ 1.0 mg/mL
Conjugate Unconjugated
Cross Reactivity

Human

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number A205
Catalog Number (CE) N/A
Size 100 µl
Price (USD) $365.00
Price (EURO) 330,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

A murine monoclonal antibody to an epitope in the C3c domain of C3.

Size 100 µl
Concentration

≥ 1.0 mg/mL

Applications See citations and technical data sheet for application info.
Form Liquid. Borate Buffered Saline (pH 8.4 ± 0.2), with ≤ 0.1% Sodium Azide.
Clonality Monoclonal
Immunogen Purified human protein.
Conjugate Unconjugated
Cross Reactivity Human
Isotype IgG1k
Purity > 95% by SDS PAGE
Source

Mouse

Specificity This monoclonal antibody was raised against purified human C3. It is specific for an antigen expressed on the C3c domain of C3 and therefore reactive to C3 and all C3c-containing fragments of C3.
Storage

Short term (30 days) 4˚C. Long term at or below –20˚C.

Background

The human complement component C3 consists of two disulfide bonded subunits (Alpha 115,000 kD and Beta 75,000 kD). The concentration of C3 in serum is approximately 1.25 +/– 0.52 mg/ml. Under normal conditions, activation of either of the complement pathways leads to C3 convertases, which cleave C3 into two fragments C3a, an anaphylatoxin, and C3b. The C3b fragment has many biologic functions including promotion of phagocytosis and participation as a structural component of both the C3 and C5 convertase enzymes. These processes are under stringent control in vivo. One control mechanism involves a two-site cleavage of C3b by Factor I with the cooperation of Factor H or CR1 as cofactors. When cleaved in this way the biologic functions of C3b are lost. The resulting protein is termed iC3b. iC3b can remain covalently bound to a target cell or bind CR3 receptors. It can be further broken down to C3c and C3d,g. 1 Quidel’s monoclonal antibodies to complement antigens were prepared using standard techniques. They are purified from mouse ascites fluid via protein A affinity chromatography. Quidel’s Monoclonal anti human C3c was raised against highly pure, human C3 using standard techniques. The specificity of the monoclonal antibody was established via a series of immunoassays utilizing highly purified C3 and C3 fragments. Firstly, the antibody was shown by ELISA to bind to purified C3, iC3b and C3c immobilized in microtiter wells, but not other complement proteins nor C3 fragments. Secondly, free (unbound) C3, iC3b, C3c and human serum but not other C3 fragments were shown (via inhibition EIA) to inhibit the binding of this antibody to immobilized C3.

Citations

Title Year Applications Sample Species Sample Sample Details

Mechanism of Borrelia immune evasion by FhbA-related proteins

2022

WB

Bacteria

Borrelia hermsii

Heme Drives Susceptibility of Glomerular Endothelium to Complement Overactivation Due to Inefficient Upregulation of Heme Oxygenase-1.

2018

FC

Cell Culture

Endothelial Cells

HUVEC Cells, HMEC Cells, HRGEC Cells

Properdin Is a Key Player in Lysis of Red Blood Cells and Complement Activation on Endothelial Cells in Hemolytic Anemias Caused by Complement Dysregulation.

2020

FC

Cell Culture

HUVEC Cells

A prevalent C3 mutation in aHUS patients causes a direct C3 convertase gain of function

2012

FC

Cell Culture

GEnCs cells

A prevalent C3 mutation in aHUS patients causes a direct C3 convertase gain of function

2012

FC

Cell Culture

HUVEC Cells

Complement factor B mutations in atypical hemolytic uremic syndrome-disease-relevant or benign?

2014

FC

Cell Culture

HUVEC Cells

Mapping interactions between complement C3 and regulators using mutations in atypical hemolytic uremic syndrome

2015

FC

Cell Culture

HUVEC Cells

Role of complement receptor 1 (CR1; CD35) on epithelial cells: A model for understanding complement-mediated damage in the kidney

2015

FC

Cell Culture

CHO Cells

Anti-inflammatory activity of intravenous immunoglobulin through scavenging of heme.

2019

FC

Cell Culture

HUVEC Cells

Complement-Mediated Selective Tumor Cell Lysis Enabled by Bi-Functional RNA Aptamers.

2021

IF

Cell Culture

MDA-MB-231 Cells

Role of membrane cofactor protein (CD46) in regulation of C4b and C3b deposited on cells

2002

FC

Hamster

CHO Cells

MCP Transfected

Polyreactive IgM initiates complement activation by PF4/heparin complexes through the classical pathway.

2018

ELISA

Human

Plasma

Platelet factor 4 (PF4)/heparin complexes

Differential and Altered Spatial Distribution of Complement Expression in Age-Related Macular Degeneration.

2021

ELISA

Human

Eye Tissue

Age-Related Macular Degeneration

Complement activation by heme as a secondary hit for atypical hemolytic uremic syndrome.

2013

FC

Human

HUVEC Cells

aHUS

Intravascular hemolysis activates complement via cell-free heme and heme-loaded microvesicles.

2018

FC

Human

HUVEC Cells

Microvesicles with heme

Functional Characterization of Autoantibodies against Complement Component C3 in Patients with Lupus Nephritis

2015

FC

Human

Endothelial Cells