C1q Protein

Purified human C1q protein


Product Specifications

Citations 31
Description

Purified human C1q protein

Storage Store at -70°C or below. Avoid freeze/thaw.
Form

Frozen liquid. 10 mM HEPES, 300 mM NaCl, pH 7.2, No preservative, 0.22 µm filtered.

Concentration 1.0 mg/mL (refer to CoA for lot specific concentration)
Applications

See citations and technical data sheet for application info.

Ordering Information

For Research Use Only. Not for use in diagnostic procedures.
Catalog Number A400
Catalog Number (CE) N/A
Size 1.0 ml
Price (USD) $275.00
Price (EURO) 245,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

Purified human C1q protein

Size 1.0 ml
Storage

Store at -70°C or below. Avoid freeze/thaw.

Form Frozen liquid. 10 mM HEPES, 300 mM NaCl, pH 7.2, No preservative, 0.22 µm filtered.
Concentration 1.0 mg/mL (refer to CoA for lot specific concentration)
Applications See citations and technical data sheet for application info.
Purity >98% by SDS PAGE
Source

Normal human serum

Activity > 1,000,000 C1H50 units/mg
Molecular Weight 410,000 Da (18 chains)
Background

Purified human complement component C1q. Circulating C1q protein is a hexamer of three unique protein subunits (A, B, and C) with molecular weights of 29, 26 and 22 kD respectively. The assembled hexamer contains a central core or stalk, 6 collagen-like domains and 6 globular protein heads. These globular or terminal regions are responsible for the binding of immunoglobulins (IgM and IgG). As part of the C1 complex (together with two copies each of C1r and C1s) C1q is central to activation of the classical complement pathway.

Citations

Title Year Applications Sample Species Sample Sample Details

Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain

2024

Liquid-liquid phase separation assay

Human

Purified Protein

Complement C1q stimulates the progression of hepatocellular tumor through the activation of discoidin domain receptor 1

2018

Cell Migration Assay

Cell Culture

HepG2 cells

Natural antibody to apoptotic cell membranes inhibits the proinflammatory properties of lupus autoantibody immune complexes

2012

Complement Activation Assay

Cell Culture

C57BL/6

Bone marrow cells

EGFR modulates complement activation in head and neck squamous cell carcinoma.

2020

IF

Cell Culture

HNSCC Cells

Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment

2008

ELISA

Hamster

CHO Cells

pEE-QuVHVL Transfected

Non-Complement-Binding De Novo Donor-Specific Anti-HLA Antibodies and Kidney Allograft Survival

2016

Binding Assay

Human

Serum

dnDSA

Complement C1q enhances homing-related responses of hematopoietic stem/progenitor cells

2010

Chemotaxis Assay

Human

Hematopoietic stem/progenitor cells

Alternative complement pathway deregulation is correlated with dengue severity

2009

ELISA

Human

Plasma

Dengue

Expression of complement components, receptors and regulators by human dendritic cells

2011

ELISA

Human

Dendritic Cell

MBL interferes with endovascular trophoblast invasion in pre-eclampsia

2012

ELISA

Human

Extravillous trophoblast cells

C6 knock-out mice are protected from thrombophilia mediated by antiphospholipid antibodies

2012

ELISA

Human

IgG Antibody

IgG-APS, IgG-MHS, IgM-APS, IgM-NHS

Evading pre-existing anti-hinge antibody binding by hinge engineering

2016

ELISA

Human

Serum

Anti-hinge antibodies

Effector-attenuating Substitutions That Maintain Antibody Stability and Reduce Toxicity in Mice

2017

ELISA

Human

C1q Protein

Binding Assay

C1q Deficiency and Neuropsychiatric Systemic Lupus Erythematosus

2016

ELISA

Human

Serum

rIgG1 Fc Hexamer Inhibits Antibody-Mediated Autoimmune Disease via Effects on Complement and FcγRs

2018

ELISA

Human

Serum

Fc activation

The Surface-Exposed Protein SntA Contributes to Complement Evasion in Zoonotic Streptococcus suis

2018

ELISA

Human

Purified protein

Number of individual ACPA reactivities in synovial fluid immune complexes, but not serum anti-CCP2 levels, associate with inflammation and joint destruction in rheumatoid arthritis

2018

ELISA

Human

Serum

Immune Complexes

Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection

2011

FC

Human

Peripheral blood mononuclear cells

SIGN-R1, a C-type lectin, enhances apoptotic cell clearance through the complement deposition pathway by interacting with C1q in the spleen

2013

FC

Human

Thymocyte Cells

Complement C1q activates tumor suppressor WWOX to induce apoptosis in prostate cancer cells

2009

IF

Human

Prostate Tissue

Dendrimer end-terminal motif-dependent evasion of human complement and complement activation through IgM hitchhiking.

2021

Protein Assay

Human

Plasma

Decidual endothelial cells express surface-bound C1q as a molecular bridge between endovascular trophoblast and decidual endothelium

2008

WB

Human

Purified protein

Complement protein C1q modulates neurite outgrowth in vitro and spinal cord axon regeneration in vivo

2015

ELISA, IP

Mouse

Brain Tissue

Cerebellum myelin substrate

Presence of plasma complement regulatory proteins clusterin (Apo J) and vitronectin (S40) on circulating immune complexes (CIC)

2006

ELISA

N/A

Purified protein

Standard curve generation

Structural characterization of a human Fc fragment engineered for lack of effector functions

2008

ELISA

N/A

C1q Protein

Local inflammation induces complement crosstalk which amplifies the antimicrobial response

2009

ELISA

N/A

C1q Protein

Mutations within a human IgG2 antibody form distinct and homogeneous disulfide isomers but do not affect Fc gamma receptor or C1q binding

2010

ELISA

N/A

CD44 (coating)

Quantitative evaluation of fucose reducing effects in a humanized antibody on Fcγ receptor binding and antibody-dependent cell-mediated cytotoxicity activities

2012

ELISA

N/A

CD-20 Antibody (AbX)

The salivary scavenger and agglutinin binds MBL and regulates the lectin pathway of complement in solution and on surfaces

2012

ELISA

N/A

rSALSA

The Natural Human IgM Antibody PAT-SM6 Induces Apoptosis in Primary Human Multiple Myeloma Cells by Targeting Heat Shock Protein GRP79

2013

ELISA

N/A

PAT-SM6 Antibody (coating)

Apoptosis signal-regulating kinase 1 mediates striatal degeneration via the regulation of C1q

2016

Complement Activation Assay

Rat

Protein Injection

Intramolecular immunological signal hypothesis revived--structural background of signalling revealed by using Congo Red as a specific tool

2015

Complement Activation Assay

Sheep

Red Blood Cells