C1q Antibody (Monoclonal)

A murine monoclonal antibody to an epitope on the globular head domain of C1q.


Product Specifications

Citations 23
Clonality

Monoclonal

Immunogen Purified human protein.
Applications See citations and technical data sheet for application info.
Concentration ≥ 1.0 mg/mL
Conjugate Unconjugated
Cross Reactivity

Human

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number A201
Catalog Number (CE) N/A
Size 100 µl
Price (USD) $365.00
Price (EURO) 330,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

A murine monoclonal antibody to an epitope on the globular head domain of C1q.

Size 100 µl
Concentration

≥ 1.0 mg/mL

Applications See citations and technical data sheet for application info.
Form Liquid. Borate Buffered Saline (pH 8.4 ± 0.2), with ≤ 0.1% Sodium Azide.
Clonality Monoclonal
Immunogen Purified human protein.
Conjugate Unconjugated
Cross Reactivity Human
Isotype IgG1k
Purity > 95% by SDS PAGE
Source

Mouse

Specificity This monoclonal antibody was raised against purified human C1q Protein. It is specific for the globular head domain.
Storage

Short term (30 days) 4˚C. Long term at or below –20˚C.

Background

Circulating C1q protein is a hexamer of three unique protein subunits (A,B, and C) with molecular weights of 29, 26 and 22 kD respectively. The assembled hexamer contains a central core or stalk, six collagen-like domains and six globular protein heads. These globular or terminal regions are responsible for the binding of immunoglobulin (IgM,and IgG). As part of the C1 complex (together with two copies each of C1r and C1s) C1q is central to activation of the classical complement pathway. The specificity of this monoclonal to purified human C1q was determined Quidel’s monoclonal antibodies to complement antigens were prepared using standard techniques. They are purified from mouse ascites fluid via protein A affinity chromatography. Quidel’s Monoclonal anti human C1q was raised against highly pure, human C1q using standard techniques. The specificity of the monoclonal antibody was established via a series of immunoassays utilizing highly purified C1q and C1q fragments. Firstly, the antibody was shown by ELISA to bind to purified C1q and C1q globular head protein immobilized in microtiter wells. Secondly, free (unbound) C1q and globular head and human serum but not other C1q fragments were shown (via inhibition EIA) to inhibit the binding of this antibody to immobilized C1q. Similarly, using radio-labeled C1q this antibody was shown to immuno-precipitate C1q using protein A-bearing bacteria.

Citations

Title Year Applications Sample Species Sample Sample Details

Mycobacterium tuberculosis modifies cell wall carbohydrates during biofilm growth with a concomitant reduction in complement activation.

2021

ELISA

Bacteria

Mycobacterium tuberculosis

Fc-Galactosylation of Human Immunoglobulin Gamma Isotypes Improves C1q Binding and Enhances Complement-Dependent Cytotoxicity.

2017

FC

Cell Culture

Raji cells

Molecular and Cellular Interactions of Scavenger Receptor SR-F1 With Complement C1q Provide Insights Into Its Role in the Clearance of Apoptotic Cells.

2020

FC

Cell Culture

THP-1 Cells

The Natural Human IgM Antibody PAT-SM6 Induces Apoptosis in Primary Human Multiple Myeloma Cells by Targeting Heat Shock Protein GRP78

2013

FC

Cell Culture

OPM-2 cells

Differential and Altered Spatial Distribution of Complement Expression in Age-Related Macular Degeneration.

2021

ELISA

Human

Eye Tissue

Age-Related Macular Degeneration

Complement Activation on Platelets Correlates with a Decrease in Circulating Immature Platelets in Patients with Immune Thrombocytopenic Purpura

2009

ELISA

Human

Plasma

Expression of complement components, receptors and regulators by human dendritic cells

2011

ELISA

Human

Dendritic Cell

Peptide Inhibitor of Complement C1 (PIC1) Rapidly Inhibits Complement Activation after Intravascular Injection in Rats

2015

ELISA

Human

C1 protein

Complement Protein C1q Binds to Hyaluronic Acid in the Malignant Pleural Mesothelioma Microenvironment and Promotes Tumor Growth

2017

ELISA

Human

Mesenchymal Cells

Serologic features of cohorts with variable genetic risk for systemic lupus erythematosus

2018

ELISA

Human

Serum

Systemic Lupus Erythematosus

Decidual endothelial cells express surface-bound C1q as a molecular bridge between endovascular trophoblast and decidual endothelium

2008

ELISA, FC

Human

Endothelial Cells

DEC, UtMEC, ADMEC, HUVEC

C1q differentially modulates phagocytosis and cytokine responses during ingestion of apoptotic cells by human monocytes, macrophages, and dendritic cells

2009

FC

Human

Jurkat Cells

C1q enhances microglial clearance of apoptotic neurons and neuronal blebs, and modulates subsequent inflammatory cytokine production

2010

FC

Human

Neuron Cells

Apoptosis

C1q enhances microglial clearance of apoptotic neurons and neuronal blebs, and modulates subsequent inflammatory cytokine production

2010

FC

Human

Neuron Cells

Imprime PGG-Mediated Anti-Cancer Immune Activation Requires Immune Complex Formation

2016

FC

Human

Neutrophils

Imprime yeast treated

Imprime PGG-Mediated Anti-Cancer Immune Activation Requires Immune Complex Formation

2016

FC

Human

Monocytes

Proteinase 3 Interferes With C1q-Mediated Clearance of Apoptotic Cells.

2018

FC, IF

Human

Red Blood Cells

Complement C1q enhances homing-related responses of hematopoietic stem/progenitor cells

2010

FC, WB

Human

Hematopoietic stem/progenitor cells

Complement activation and increased expression of Syk, mucin-1 and CaMK4 in kidneys of patients with COVID-19.

2021

IF

Human

Kidney Tissue

COVID-19

Pentraxin 3 recruits complement factor H to protect against oxidative stress-induced complement and inflammasome overactivation.

2016

WB

Human

ARPE-19 Cells

C1q propagates microglial activation and neurodegeneration in the visual axis following retinal ischemia/reperfusion injury.

2016

IF

Mouse

Retina Tissue

Proteinase 3 Interferes With C1q-Mediated Clearance of Apoptotic Cells.

2018

FC, IF

Rat

Basophilic Leukemia cells

Activation of early components of complement targets myelin and oligodendrocytes in the aged rhesus monkey brain.

2005

IHC

Rhesus Monkey

Brain Tissue