C4c Antibody (Monoclonal) , Biotinylated

A biotin conjugated murine monoclonal antibody to an epitope in the C4c domain of C4.


Product Specifications

Citations0
Clonality

Monoclonal

Immnogen

Purified human protein.

Applications

See citations and technical data sheet for application info.

Concentration0.2 mg/mL
ConjugateBiotin
Cross Reactivity

Human, Baboon

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog NumberA703
Catalog Number (CE)N/A
Size250 µL
Price (USD)$365.00
Price (EURO)330,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

A biotin conjugated murine monoclonal antibody to an epitope in the C4c domain of C4.

Size

250 µL

Concentration0.2 mg/mL
ApplicationsSee citations and technical data sheet for application info.
FormLiquid. Phosphate Buffered Saline (pH 7.0 ± 0.2), 1% Bovine Serum Albumin, with ≤ 0.1% Sodium Azide.
ClonalityMonoclonal
Immunogen

Purified human protein.

ConjugateBiotin
Cross ReactivityHuman, Baboon
Isotype

IgG1k

Purity

≥ 95% by SDS PAGE

SourceMouse
Specificity

This monoclonal antibody was raised against purified human C4. It is specific for an antigen expressed on the C4c domain of C4 and therefore reactive to C4 and all C4c-containing fragments of C4.

Storage

Short term (30 days) 4˚C. Long term at or below –20˚C.

Background

Activation of the classical complement pathway begins with the binding of an activating substance (e.g. immune complex) to the C1q molecule of C1. This, in turn, activates the C1r(2)C1s(2) sub-units, resulting in cleavage of C4 to C4b near the amino terminus of the gamma chain releasing C4a in the process. The short-lived C4b molecule can bind covalently to membranes or other surfaces via either an amide or ester bond. This is an inefficient process that is limited to the immediate vicinity of the C1 complex. C4b then takes part in the classical convertase enzyme. Because of the short life of the C4b molecule much of the C4d is free and circulates in serum. Both bound and free C4b are strictly controlled in vivo. The ability of C4b to participate in classical pathway activation and opsonization reactions is inhibited by a single site cleavage of the alpha chain by Factor I. This reaction requires either C4 binding protein or CR1 as a cofactor. This initial cleavage inactivates C4b resulting in iC4b. Further degradation of this molecule by Factor I produces the C4c and C4d fragments. Both of these fragments can be produced in fluid phase or on target surface.

Citations

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