C1s Protein

Purified human C1s protein


Product Specifications

Citations0
Description

Purified human C1s protein

Storage

Store at -70°C or below. Avoid freeze/thaw.

Form

Frozen liquid. 50 mM sodium phosphate, 130 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered.

Concentration

1.0 mg/mL (refer to CoA for lot specific concentration)

Applications

See citations and technical data sheet for application info.

Ordering Information

For Research Use Only. Not for use in diagnostic procedures.
Catalog NumberA425
Catalog Number (CE)N/A
Size250 µL
Price (USD)$285.00
Price (EURO)220,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

Purified human C1s protein

Size250 µL
Storage

Store at -70°C or below. Avoid freeze/thaw.

Form

Frozen liquid. 50 mM sodium phosphate, 130 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered.

Concentration1.0 mg/mL (refer to CoA for lot specific concentration)
ApplicationsSee citations and technical data sheet for application info.
Purity>90 % by SDS PAGE
SourceNormal human serum
Activity>90 % C1s will bind with excess C1-INH
Molecular Weight86,000 Da (2 chains)
Background

C1s enzyme is the activated form of C1s proenzyme. C1s is a subunit of the C1 complex which is the first complement component in the classical pathway of complement. C1s proenzyme is an inactive zymogen until C1 is activated. C1s proenzyme is activated when C1 binds to and is activated by antibodies bound to antigens (immune complexes) yielding C1r enzyme, the first protease that initiates the cascade. C1r enzyme in the C1 complex activates C1s proenzyme generating C1s enzyme. C1 complex is a non-covalent calcium-dependent complex of one C1q, two C1r and two C1s molecules. C1q binds by two or more of its six arms to the Fc domains of IgG or IgM. The binding of multiple arms to immune complexes causes the two C1r proteins in the complex (protease zymogens) to activate producing two proteases that cleave and activate the two C1s proenzymes in the complex. This activation of C1s proenzyme results in its cleavage into the two chain C1s enzyme with 58,000 and 28,000 dalton fragments. Activated C1s enzyme cleaves complement component C4 releasing C4a and initiating covalent attachment of C4b to the activating surface. Activated C1s also cleaves C2 and the larger fragment of C2 binds to the surface-attached C4b forming C4b,C2a, the C3/C5 convertase of the classical pathway.

Citations

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