C4/C4c Antibody (Monoclonal)

A murine monoclonal antibody to an epitope in the C4c domain of C4.


Product Specifications

Citations 14
Clonality

Monoclonal

Immunogen Purified human protein.
Applications See citations and technical data sheet for application info.
Concentration 1.0-1.2 mg/mL
Conjugate Unconjugated
Cross Reactivity

Human, Baboon

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number A211
Catalog Number (CE) N/A
Size 100 µl
Price (USD) $365.00
Price (EURO) 330,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

A murine monoclonal antibody to an epitope in the C4c domain of C4.

Size 100 µl
Concentration

1.0-1.2 mg/mL

Applications See citations and technical data sheet for application info.
Form Liquid. Borate Buffered Saline (pH 8.4 ± 0.2), with ≤ 0.1% Sodium Azide.
Clonality Monoclonal
Immunogen Purified human protein.
Conjugate Unconjugated
Cross Reactivity Human, Baboon
Isotype IgG1k
Purity ≥ 95% by SDS PAGE
Source

Mouse

Specificity This monoclonal antibody was raised against purified human C4. It is specific for an antigen expressed on the C4c domain of C4 and therefore reactive to C4 and all C4c-containing fragments of C4.
Storage

Short term (30 days) 4˚C. Long term at or below –20˚C.

Background

Activation of the classical complement pathway begins with the binding of an activating substance (e.g. immune complex) to the C1q molecule of C1. This, in turn, activates the C1r(2)C1s(2) sub-units, resulting in cleavage of C4 to C4b near the amino terminus of the gamma chain releasing C4a in the process. The short-lived C4b molecule can bind covalently to membranes or other surfaces via either an amide or ester bond. This is an inefficient process that is limited to the immediate vicinity of the C1 complex. C4b then takes part in the classical convertase enzyme. Because of the short life of the C4b molecule much of the C4d is free and circulates in serum. Both bound and free C4b are strictly controlled in vivo. The ability of C4b to participate in classical pathway activation and opsonization reactions is inhibited by a single site cleavage of the alpha chain by Factor I. This reaction requires either C4 binding protein or CR1 as a cofactor. This initial cleavage inactivates C4b resulting in iC4b. Further degradation of this molecule by Factor I produces the C4c and C4d fragments. Both of these fragments can be produced in fluid phase or on target surface.

Citations

Title Year Applications Sample Species Sample Sample Details

Human pentraxin 3 binds to the complement regulator c4b-binding protein

2011

FC

Cell Culture

Jurkat Cells

Role of complement receptor 1 (CR1; CD35) on epithelial cells: A model for understanding complement-mediated damage in the kidney

2015

FC

Cell Culture

CHO Cells

Role of membrane cofactor protein (CD46) in regulation of C4b and C3b deposited on cells

2002

FC

Hamster

CHO Cells

MCP Transfected

Membrane cofactor protein mutations in atypical hemolytic uremic syndrome (aHUS), fatal Stx-HUS, C3 glomerulonephritis, and the HELLP syndrome

2008

FC

Hamster

CHO Cells

MCP Transfected

Expression of complement components, receptors and regulators by human dendritic cells

2011

ELISA

Human

Dendritic Cell

Complement C1q-dependent excitatory and inhibitory synapse elimination by astrocytes and microglia in Alzheimer's disease mouse models.

2023

ELISA

Human

CSF

Alzheimer's Disease

Binding of Complement Inhibitor C4b-binding Protein to a Highly Virulent Streptococcus Pyogenes M1 Strain Is Mediated by Protein H and Enhances Adhesion to and Invasion of Endothelial Cells.

2013

FC

Human

Serum

S. pyogenes incubated

Binding of C4b-binding protein to porin: a molecular mechanism of serum resistance of Neisseria gonorrhoeae

2001

FC

Human

Serum

Neisseria gonorrhoeae

Evasion of Phagocytosis through Cooperation between Two Ligand-binding Regions in Streptococcus pyogenes M Protein.

2003

FC

Human

Serum

S. pyrogenes incubated

Binding of vitronectin by the Moraxella catarrhalis UspA2 protein interferes with late stages of the complement cascade

2006

FC

Human

Serum

Moraxella catarrhalis

Binding of vitronectin by the Moraxella catarrhalis UspA2 protein interferes with late stages of the complement cascade

2006

FC

Human

Serum

Neisseria gonorrhoeae

Heptose I glycan substitutions on Neisseria gonorrhoeae lipooligosaccharide influence C4b-binding protein binding and serum resistance

2007

FC

Human

Serum

Neisseria gonorrhoeae

SLE serum deposits C4d on red blood cells, decreases red blood cell membrane deformability, and promotes nitric oxide production

2011

FC

Human

Serum

Systemic Lupus Erythematosus

Decidual endothelial cells express surface-bound C1q as a molecular bridge between endovascular trophoblast and decidual endothelium

2008

IHC

Human

Endothelium Tissue