Lyra® Direct C. difficile Assay
Lyra® Assays
Lyra® Direct C. difficile Assay

The Lyra Direct C. difficile Assay is a qualitative, multiplexed in vitro  diagnostic test for the detection of toxin A gene (tcdA) or toxin B gene (tcdB) sequences of toxigenic strains of Clostridium difficile  from unformed (liquid or soft) stool specimens collected from patients suspected of having Clostridium difficile -associated disease (CDAD).

A multiplex real-time PCR reaction is performed under optimized conditions in a single well generating amplicons for each of the targets present in the sample. Identification occurs using oligonucleotide primers and probes that are complementary to conserved regions in the  tcdA  and tcdB  genes of the pathogenicity locus.

The Lyra Direct C. difficile Assay has one-step reagent set-up and a three-step sample prep. Test results are available in less than 70 minutes. Lyra Direct C. difficile is a quality real-time PCR available for use on Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument, QuantStudioTM Dx Real-Time PCR Instrument and Cepheid® SmartCycler® II.

The Lyra Direct C. difficile test is one in a growing menu of real-time PCR assays and intended to aid in the diagnosis of CDAD in humans. 

 

Please see chart below for ordering information.

Contact us

 

Lyra® Assays
Lyra® Direct C. difficile Assay
Lyra® Direct C. difficile Assay

The Lyra Direct C. difficile Assay is a qualitative, multiplexed in vitro  diagnostic test for the detection of toxin A gene (tcdA) or toxin B gene (tcdB) sequences of toxigenic strains of Clostridium difficile  from unformed (liquid or soft) stool specimens collected from patients suspected of having Clostridium difficile -associated disease (CDAD).

A multiplex real-time PCR reaction is performed under optimized conditions in a single well generating amplicons for each of the targets present in the sample. Identification occurs using oligonucleotide primers and probes that are complementary to conserved regions in the  tcdA  and tcdB  genes of the pathogenicity locus.

The Lyra Direct C. difficile Assay has one-step reagent set-up and a three-step sample prep. Test results are available in less than 70 minutes. Lyra Direct C. difficile is a quality real-time PCR available for use on Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument, QuantStudioTM Dx Real-Time PCR Instrument and Cepheid® SmartCycler® II.

The Lyra Direct C. difficile test is one in a growing menu of real-time PCR assays and intended to aid in the diagnosis of CDAD in humans. 

 

Please see chart below for ordering information.

Contact us

 

Ordering information
Catalog number Description Kit size / Case size
M105 Assay Kit 96 tests
M207 Sample Preparation Kit 96 tests
M108 Quidel Molecular C. difficile Control Set 40 reactions

 

Features & benefits

Rehydration solution is simply added to the lyophilized Master Mix for one-step reagent set-up

Simplified and uniform workflow with standard pipetting volumes for easy training

Long shelf life — two years from date of manufacture

Find what you need

To access safety data sheets, package inserts, quick reference guides and all other technical and promotional materials, visit our technical documents library.

 

Technical documentation

Product specification
Sample typeUnformed (liquid or soft) stool samples
Time to resultsLess than 70 minutes
Reagent storage conditions2°C to 8°C
Controls storage conditions2°C to 8°C
Sample preparation storage conditions2°C to 25°C
Sensitivity*94.3% AB 7500 Fast Dx, 93.3 % QuantStudio Dx; 89.7% Smart Cycler II
Specificity*94.2% AB 7500 Fast Dx; 93.4% QuantStudio Dx; 93.3% SmartCycler II
LOD0.2 to 1 CFU
Processed sample needed to test5 µL
Shelf life24 months

 

*Refer to Package Insert for additional performance claims.

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