Factor B Antibody (Polyclonal)

A goat antiserum raised against human Factor B.


Product Specifications

Citations 18
Clonality

Polyclonal

Immunogen Highly purified human Factor B protein
Applications See citations and technical data sheet for application info.
Concentration > 40 mg/mL
Conjugate Unconjugated
Cross Reactivity

Human, Baboon, Horse, Dog, Cat, Rabbit, Hamster, Mouse, Rat, Guinea Pig, Swine

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog Number A311
Catalog Number (CE) N/A
Size 2.0 mL
Price (USD) $195.00
Price (EURO) 170,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

A goat antiserum raised against human Factor B.

Size 2.0 mL
Concentration

> 40 mg/mL

Applications See citations and technical data sheet for application info.
Form Liquid. Whole Antiserum. ≤ 0.1% Sodium Azide
Clonality Polyclonal
Immunogen Highly purified human Factor B protein
Conjugate Unconjugated
Cross Reactivity Human, Baboon, Horse, Dog, Cat, Rabbit, Hamster, Mouse, Rat, Guinea Pig, Swine
Isotype Goat IgG
Purity N/A
Source

Goat

Specificity The Anti-Human Factor B polyclonal antisera was tested by double-immunodiffusion, one-dimensional immunoelectrophoresis, quantitative radial immunodiffusion, and quantitative rocket immunoelectrophoresis. The antisera was determined to be monospecific for the Factor B protein.
Storage

Short term (30 days) 4˚C. Long term at or below –20˚C.

Background

Primarily synthesized in the liver, Factor B is a plasma protein that is unique to the alternative pathway of the Complement system. The average concentration of Factor B in human serum/plasma is approximately 200 µg/mL. Factor B present in the circulatory system is a single-chain polypeptide with a molecular weight of 93 KD. Factor B will bind to the C3b component of the complement system in the presence of Mg++ to form the C3bB complex. This complex can be activated through cleavage of the Factor B portion by Factor D, which releases the Ba fragment of Factor B while the Bb fragment of Factor B remains bound to C3b. The C3bBb complex acts as a C3 and C5 convertase due to the serine protease activity of the Bb fragment. Presence of the split products (Ba and Bb fragments of Factor B) indicate that the alternative complement pathway has been activated. Factor B, like C2 and C4 is characterized as a Class III major histocompatibility complex (MHC) antigen. The structural gene coding for this protein is located within the MHC on chromosome 6 in man, and chromosome 17 in mice.

Citations

Title Year Applications Sample Species Sample Sample Details

FACIN, a Double-Edged Sword of the Emerging Periodontal Pathogen Filifactor Alocis: A Metabolic Enzyme Moonlighting as a Complement Inhibitor.

2016

WB

Bacteria

Filifactor alocis

CipA mediates complement resistance of Acinetobacter baumannii by formation of a factor I-dependent quadripartite assemblage.

2022

WB

Bacteria

A. baumannii

Complement activation in pediatric patients with recurrent acute otitis media

2013

IF, WB

Cell Culture

HMEE Cells

The Alternative Pathway Is Necessary and Sufficient for Complement Activation by Anti-THSD7A Autoantibodies, Which Are Predominantly IgG4 in Membranous Nephropathy.

2022

ELISA

Human

Serum

α-2,3-sialyltransferase expression level impacts the kinetics of lipooligosaccharide sialylation, complement resistance, and the ability of Neisseria gonorrhoeae to colonize the murine genital tract.

2015

ELISA

Human

Serum

N. gonorrhoeae incubated

Variability and action mechanism of a family of anticomplement proteins in Ixodes ricinus

2008

ELISA, WB

Human

Red Blood Cells

Ixodes ricinus incubated

Pentraxin 3 recruits complement factor H to protect against oxidative stress-induced complement and inflammasome overactivation.

2016

WB

Human

ARPE-19 cells

Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina.

2021

WB

Human

Retina Tissue

Expression of complement components, receptors and regulators by human dendritic cells

2011

WB

Human

Monocyte Derived Dendritic Cell

Essential role of factor B of the alternative complement pathway in complement activation and opsonophagocytosis during acute pneumococcal otitis media in mice

2011

IF, WB

Mouse

Bone Tissue

Temporal Bone

Essential role of factor B of the alternative complement pathway in complement activation and opsonophagocytosis during acute pneumococcal otitis media in mice

2011

IF, WB

Mouse

Middle Ear Fluid

Cytokine and Complement Response in the Glaucomatous βB1-CTGF Mouse Model.

2021

IHC

Mouse

Eye Tissue

C3(H2O) prevents rescue of complement-mediated C3 glomerulopathy in Cfh-/- Cfd-/- mice

2020

WB

Mouse

Plasma

Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina.

2021

WB

Mouse

Eye Tissue

Retina, Sodium Iodate-Induced Degeneration

Complement factor B is critical for sub-RPE deposit accumulation in a model of Doyne honeycomb retinal dystrophy with features of age-related macular degeneration.

2022

WB

Mouse

Eye Tissue

C3 dysregulation due to factor H deficiency is mannan-binding lectin-associated serine proteases (MASP)-1 and MASP-3 independent in vivo

2014

WB

Mouse

Plasma

Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina

2016

WB

Mouse

Plasma

Efficacy of GalNAc C3 siRNAs in factor H-deficient mice with C3 glomerulopathy

2024

WB

Mouse

Plasma

C3G