C3 Protein
Purified human C3 protein
Product Specifications
Citations | 17 |
---|---|
Description |
Purified human C3 protein |
Storage | Store at -70°C or below. Avoid freeze/thaw (>10 % activity loss per thaw). |
Form |
Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered. |
Concentration | 1.0 mg/mL (refer to CoA for lot specific concentration) |
Applications |
See citations and technical data sheet for application info. |
Ordering Information
Catalog Number | A401 |
---|---|
Catalog Number (CE) | N/A |
Size | 250 µL |
Price (USD) | $250.00 |
Price (EURO) | 220,00 € |
Contact us
US Phone | +1 (858) 552 1100 |
---|---|
EU Phone | +353 (91) 412 474 |
US Email | contact-us@quidelortho.com |
EU Email | contact-emea@quidelortho.com |
- Specifications
- Citations
- Certificate of Analysis
Specifications
Description |
Purified human C3 protein |
---|---|
Size | 250 µL |
Storage |
Store at -70°C or below. Avoid freeze/thaw (>10 % activity loss per thaw). |
Form | Frozen liquid. 10 mM sodium phosphate, 145 mM NaCl, pH 7.2. No preservative, 0.22 µm filtered. |
Concentration | 1.0 mg/mL (refer to CoA for lot specific concentration) |
Applications | See citations and technical data sheet for application info. |
Purity | >95% by SDS-PAGE |
Source |
Normal human serum |
Activity | >70% versus normal human serum standard |
Molecular Weight | 185,000 Da (2 chains) |
Background |
Purified human complement component C3. The third component of complement is a centrally important constituent of both the alternative and classical pathways. Upon secretion, pro-C3 undergoes a site specific proteolytic cleavage to yield circulating C3, a protein containing two disulfide bonded subunits (Alpha 115 kD and Beta 75 kD). The concentration of C3 in serum is approximately 1.25 +/– 0.52 mg/mL. |
Citations
Title | Year | Applications | Sample Species | Sample | Sample Details |
---|---|---|---|---|---|
Complement-Mediated Selective Tumor Cell Lysis Enabled by Bi-Functional RNA Aptamers. |
2021 | Binding Assay |
Cell Culture |
MDA-MB-231 Cells |
|
2022 | Complement Activation Assay |
Human |
HBE Cells |
Cigarette smoke-induced oxidative stress |
|
Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo. |
2017 | Dot Blot |
Human |
Serum |
SPIO nanoworms |
Alternative complement pathway deregulation is correlated with dengue severity |
2009 | ELISA |
Human |
Plasma |
Dengue |
Expression of complement components, receptors and regulators by human dendritic cells |
2011 | ELISA |
Human |
Dendritic Cell |
|
2011 | ELISA |
Human |
Peripheral blood mononuclear cells |
||
Properdin binding to complement activating surfaces depends on initial C3b deposition |
2017 | ELISA |
Human |
Serum |
|
Extracellular vesicles from KSHV-infected endothelial cells activate the complement system |
2017 | ELISA |
Human |
HUVEC Cells |
KSHV infection |
2018 | Silver Stain |
Human |
Purified protein |
B. lanceolatus venom incubated |
|
2014 | WB |
Human |
Serum |
||
2015 | ELISA |
Mouse |
Brain Tissue |
Cerebellum myelin substrate |
|
Complement Protein C3 Suppresses Axon Growth and Promotes Neuron Loss |
2017 | ELISA |
Mouse |
Neuron Cells |
|
2006 | ELISA |
N/A |
Purified protein |
Standard curve generation |
|
2021 | WB |
N/A |
Control |
||
1996 | WB |
N/A |
C3 Protein |
||
2015 | Silver Stain |
Premolis semirufa (Caterpillar) |
Bristle Extract |
||
2013 | Silver Stain |
Snake |
Bothrops pirajai venom |