Lyra® Influenza A+B Assay
Lyra® Assays
Lyra® Influenza A+B Assay

The Lyra Influenza A+B assay is a multiplex real-time RT-PCR assay for the qualitative detection and identification of viral nucleic acids extracted from nasal swabs and nasopharyngeal swabs specimens. The real-time influenza test has been shown to detect HIN1, H3N2 and H7N9 subtypes but does not differentiate the subtypes from Influenza A.

A multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of influenza A occurs using target specific primers and a fluorescent-labeled probe that hybridizes to a conserved region within the matrix protein gene. Identification of influenza B occurs using target specific primers and fluorescent-labeled probes that hybridize to a conserved influenza B sequence within the neuraminidase gene.

The Lyra Influenza A+B assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra Influenza A+B RT-PCR is available for use on the Applied Biosystems®  7500 Fast Dx Real-Time PCR Instrument, QuantStudioTM  Dx Real-Time PCR Instrument and Cepheid® SmartCycler®  II.

The Lyra Influenza A+B assay is one of a growing menu of assays and is intended to aid in the differential diagnosis of influenza A and/or influenza B viral infections in humans. 

Please see chart below for ordering information.

Contact us

 

Lyra® Assays
Lyra® Influenza A+B Assay
Lyra® Influenza A+B Assay

The Lyra Influenza A+B assay is a multiplex real-time RT-PCR assay for the qualitative detection and identification of viral nucleic acids extracted from nasal swabs and nasopharyngeal swabs specimens. The real-time influenza test has been shown to detect HIN1, H3N2 and H7N9 subtypes but does not differentiate the subtypes from Influenza A.

A multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of influenza A occurs using target specific primers and a fluorescent-labeled probe that hybridizes to a conserved region within the matrix protein gene. Identification of influenza B occurs using target specific primers and fluorescent-labeled probes that hybridize to a conserved influenza B sequence within the neuraminidase gene.

The Lyra Influenza A+B assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra Influenza A+B RT-PCR is available for use on the Applied Biosystems®  7500 Fast Dx Real-Time PCR Instrument, QuantStudioTM  Dx Real-Time PCR Instrument and Cepheid® SmartCycler®  II.

The Lyra Influenza A+B assay is one of a growing menu of assays and is intended to aid in the differential diagnosis of influenza A and/or influenza B viral infections in humans. 

Please see chart below for ordering information.

Contact us

 

Ordering information
Catalog numberDescriptionKit size / Case size
M100 Assay Kit 96 tests
M106 Quidel Molecular Influenza A+B Control Set 10 reactions

 

Features & benefits

Rehydration solution is simply added to the lyophilized Master Mix for one-step reagent set-up.

Speedy results in less than 75 minutes after extraction.

Simplified and uniform workflow with standard pipetting volumes for easy training.

Find what you need

To access safety data sheets, package inserts, quick reference guides and all other technical and promotional materials, visit our technical documents library.

 

Technical documentation

Product specifications
Sample type Nasal swab, Nasopharyngeal swab
Time to results Less than 75 minutes after extraction
Reagent storage conditions 2°C to 8°C
Controls storage conditions 2°C to 8°C
Positive Percent Agreement* A: 100%; B: 98.4%
Negative Percent Agreement* A: 98.7%; B: 95.5%
LOD 2 to 100 TCID50/mL
Sample volume needed for extraction 180 µL
Purified nucleic acid volume needed to testing 5 µL
Shelf life 24 months

 

*Refer to Package Insert for additional performance claims.

 

 

 

 

 

Workflow using the 7500 series

s-card-img

Related products

Solana® Influenza A+B Assay

Solana® Respiratory Viral Panel

QuickVue® Influenza A+B Test

Sofia® 2 Flu + SARS Antigen FIA

Sofia® Influenza A+B FIA

D3® Double Duet DFA Respiratory Virus Screening & ID Kit

D3® Duet DFA Influenza A/Respiratory Virus Screening Kit

D3® Duet DFA RSV/Respiratory Virus Screening Kit

D3® Ultra 8TM DFA Individual Reagent Sets

Influenza