Mouse Factor B Antibody (FB/Bb)

A rat monoclonal antibody to an epitope on mouse Factor Bb protein.


Product Specifications

Citations0
Clonality

Monoclonal

Immnogen

Recombinant mouse Factor B protein immunized in rat.

Applications

ELISA, WB (See citations and technical data sheet for application info)

Concentration~1 mg/mL. Varies by lot so refer to CoA.
ConjugateUnconjugated
Cross Reactivity

None

Ordering Information

For Research Use Only in the United States. Not for use in diagnostic procedures.
Catalog NumberA802
Catalog Number (xUS)N/A
Size100 µg
Price (USD)$350.00
Price (EURO)320,00 €

Contact us

US Phone+1 (858) 552 1100
EU Phone+353 (91) 412 474
US Emailcontact-us@quidelortho.com
EU Emailcontact-emea@quidelortho.com

Specifications

Description

A rat monoclonal antibody to an epitope on mouse Factor Bb protein.

Size

100 µg

Concentration~1 mg/mL. Varies by lot so refer to CoA.
ApplicationsELISA, WB (See citations and technical data sheet for application info)
FormLiquid. PBS, pH 7.2
ClonalityMonoclonal
Immunogen

Recombinant mouse Factor B protein immunized in rat.

ConjugateUnconjugated
Cross ReactivityNone
Isotype

IgG2bκ

Purity

≥ 85% by SDS Page

SourceRat
Specificity

The specificity of the monoclonal antibody was established via a series of immunoassays. The antibody was shown by ELISA and Western Blot (WB) to bind highly purified mouse intact Factor B protein and fragment Bb, but not fragment Ba.

StorageShort term (30 days) 4˚C. Long term at or below –20˚C
Background

Factor B is one of the complement proteins which is unique to the Alternative Pathway. It is a single-chain glycoprotein with a molecular weight of approximately 98 kD. The concentration of Factor B in human serum/plasma is ~200 μg/mL. The Alternative Complement Pathway provides innate protection against microbial agents in the absence of specific antibodies. The activation of this complement pathway can be triggered by a variety of substances, including microbial polysaccharaides or lipids, gram-negative bacterial lipopolysaccharides, and surface determinants present on some viruses, parasites, virally infected mammalian cells, and cancer cells. In autoimmune diseases, the Alternative Complement Pathway may contribute directly to tissue damage. A centrally important reaction that occurs during alternative pathway activation is the conversion of the 93 kD molecular weight Factor B zymogen to an active proteolytic enzyme. This is accomplished in a two-step reaction. During the first reaction step, Factor B forms a magnesium-dependent complex with C3(H2O) or C3b. The C3(H2O),B complex is formed only in fluid phase, while the C3b,B complex can be formed either in fluid phase or on a target surface. Factor B, present in either complex, is cleaved into the Ba (33 kD) and Bb (60 kD) fragments in the second reaction step by the Alternative Pathway enzyme, Factor D. The C3b,Bb bimolecular complex constitutes the C3 convertase enzyme of the Alternative Pathway, while the Ba fragment is released into the fluid phase.

Citations

TitleYearApplicationsSample SpeciesSampleSample Details